Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
نویسندگان
چکیده
منابع مشابه
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples.
Here we outline a next-generation RNA sequencing protocol that enables de novo assemblies and intra-host variant calls of viral genomes collected from clinical and biological sources. The method is unbiased and universal; it uses random primers for cDNA synthesis and requires no prior knowledge of the viral sequence content. Before library construction, selective RNase H-based digestion is used...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2016
ISSN: 1940-087X
DOI: 10.3791/54117